Aug 20, 2008 · Paired end (mate pair) sequencing explanation biocc, "paired end" or "mate pair" refers to how the library is made, and then how it is sequenced. Both are methodologies that, in addition to …

Nov 18, 2011 · Mate-pair is a specific type of library; paired-end is a type of sequencing. They are not two different methods; in fact, mate-pair libraries require paired-end sequencing. The decision to use …

Aug 3, 2011 · From these 65 million reads, doing a paired end sequencing run vs a single end run means that you will get 65 million pairs (or 130 million reads) vs 65 million single end reads. …

Aug 15, 2011 · 50 base-pair paired-end reads span a longer region of the transcript. Each read represents one end of a ~200-300 base-pair RNA fragment, compared to a 100 base-pair read which …

Jul 16, 2015 · Paired-End Sequencing from MiSeq 07-16-2015, 12:49 PM Hi guys, I am a newbie for Illumina sequencing. I just got my sequencing results back from a sequencing center. I am doing …

Sep 26, 2017 · The data was generated by 10x genomics library prep (not sure of the specific microfluidic hardware) followed by sequencing on an Illumina machine. The project PI requested a …

Jul 31, 2011 · The insert is normally the stretch of sequence between the paired-end adapters, so in your case the insert size would be 250 bp (2x75 bp reads + 100 bp unsequenced middle piece). The …

Jun 9, 2014 · If I understand correctly, the library is not actually a small RNA library. In sample sheet you can put library type as TruSeq DNA library and that should allow paired end sequencing.

Jan 27, 2011 · Hello all, I am new to RNA-seq analysis, trying to decide whether to do single-end or paired-end RNA seq for the purpose of differential expression and alternative splicing. We use the …

Tweet #10 08-21-2012, 09:56 PM and, if the paired-end read sequencing result anyway comes from 1 fragment with various length (for example, 150~500bp), the aligned position of two read (1 forward …